A canção do PCR

Muitos já terão ouvido o popular Hino aos Funcionários Públicos de Portimão, mas não conhecerão a canção do PCR (Polymerase Chain Reaction), uma técnica que é uma espécie de máquina de fotocópias de ADN, pelo qual foi atribuído o Nobel da Química em 1993.

Oiça e delicie-se com a biologia molecular!

There was a time when to amplify DNA,

You had to grow tons and tons of tiny cells.

(Oooh) Then along came a guy named Dr. Kary Mullis,

Said you can amplify in vitro just as well.

Just mix your template with a buffer and some primers,

Nucleotides and polymerases too.

Denaturing, annealing, and extending,

Well it’s amazing what heating and cooling and heating will do.

PCR when you need to detect mutation (detect mutation)

PCR when you need to recombine (recombine)

PCR when you need to find out who the daddy is (who’s your daddy?)

PCR when you need to solve a crime (solve a crime)